Hydrophilic Polymer Exclusion Type ODS Column
Sinking ODS
FEATURES
World's first: Hydrophilic/Hydrophobic Hybrid ODS Column
Direct injection of serum (plasma)
Hydrophilic polymer exclusion/Hydrophobic small molecule adsorption
Analysis of drugs in liquid formulations
LC-MS compatible
Wide range of column sizes: Inner diameter 0.075 - 10mm / Length 10-250mm
MAIN SPECIFICATIONS
| Particle size: | 3μm |
| Base materials: | Fully porous, high-purity silica gel |
| Ligand: | Octadecyl group, hydrophilic group |
| Target molecular weight: | Exclusion above Ca. 30kDa, adsorption below |
| pH range: | pH 2-8 |
| Maximum pressure: | 250bar / 3,500psi / 25MPa |
| USP Code: | L1 |
There have been existing columns for directly analyzing drugs in serum, each with the following drawbacks: 1. Pre-treatment columns for column switching complicate LC system configuration. 2. Isocratic analysis columns constrained by organic solvent concentration. 3. LC-MS incompatible columns requiring phosphate buffer in the mobile phase.
Cadenza HS-C18 is the world's first hydrophilic/hydrophobic hybrid ODS column capable of direct serum injection. It excludes high molecular weight serum proteins like albumin from the packing pores, while hydrophobically retaining low molecular weight drugs within the pores and separating them by organic solvent gradient elution.
Simplifying serum pretreatment processes, it is anticipated to be applied in the field of Therapeutic Drug Monitoring (TDM) where rapid analysis is crucial. Recently, it has been widely used to control surfactant concentrations, stabilizers of monoclonal antibody formulations in high demand. Additionally, this product effectively separates hydrophilic polymers other than proteins and drugs in liquid formulations such as injectables.
Cadenza HS-C18 enables LC-MS analysis of small molecules in protein and hydrophilic polymer solutions, expected to be widely adopted in various fields including blood analysis and formulation analysis.
Technical Information
Applications NEW
Latest Applications
- ● Surfactants in Antibody Aqueous Solutions
- ● Minoxidil in Serum NEW
- ● Antiepileptic Drugs in Bovine Serum
- ● Perfluoroalkyl Acids in Bovine Serum
- ● Dizopyr-Amine in Povidone Solution
- ● Verapamil in HPC Solution
- ● Lidocaine in CMC Solution
- ● Sulfamethoxazole in Sodium Alginate Solution
- ● Calcitonin (Peptide) in Gelatin (Protein) Solution
User Reports
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●Analysis of Surfactants in Monoclonal Antibody (mAb) Formulations
Amgen, USA
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●LC-MS/MS Rapid Quantitative Method for Antiepileptic Drugs (AEDs) in Plasma
Inha University, KOREA
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●Non-targeted Metabolomics by LC-QTOF MS
Korea Institute of Science and Technology, KOREA
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●Development of a Novel Micafungin Plasma Concentration Measurement Method by Direct Serum Injection
Gifu Pharmaceutical University, JAPAN
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●Practicality of Plasma Direct Injection Method
Toa Pharmaceutical, JAPAN