IMTAKT Intrada Amino Acid Analysis Column for LC-MS

Life Sciences Separation Column

World's First LC-MS Untargeted Amino Acid Analysis Column

IMTAKT Intrada Amino Acid Analysis Column for LC-MS

FEATURES

Enables direct LC-MS analysis of amino acids without derivatization

Capable of separating isomers like Leu, Ile, and others

Protein constituent amino acids can be measured in 5-10 minutes

Suitable for amino acids, related compounds, dipeptides, etc.

High-throughput analysis in less than 1 minute possible depending on amino acid composition

The conventional methods of amino acid analysis had the following difficulties.

  • Post-labeling Methods: Utilizes ion-exchange modes, requiring complete separation of amino acids, leading to complex mobile phases and difficulties in shortening analysis time.
  • Pre-labeling Methods: Allows for short-time analysis using reversed-phase modes, but requires time and equipment for amino acid pre-labeling reactions. Additionally, labeling efficiency is susceptible to sample matrix interference.

Advantages of this Product

Our product allows selective detection of amino acids with different masses using LC-MS. As a pioneering column specialist, we have developed a dedicated stationary phase surface structure, a world-first, to leverage universal LC-MS.

Intrada Amino Acid column, coupled with universal LC-MS, enables rapid analysis without the complexity of labeling. Moreover, with unprecedented column size variations, it achieves high-throughput analysis in as little as 1 minute depending on amino acid compositions.

Its innovative design not only separates Leucine/Isoleucine but also accommodates a wide range of applications for amino acid-related compounds such as β- and γ-amino acid isomers and dipeptides.

PUBLICATIONS

U.S. FDA Publication

Real-Time Quantification of Amino Acids in Antibody Drug Production Media

Real-time quantification and supplementation of bioreactor amino acids to prolong culture time and maintain antibody product quality
https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btpr.2894

Tokyo Customs Laboratory Report No. 60

Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)

Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)
https://www.customs.go.jp/ccl_search/info_search/foodstuffs/r_60_03_j.pdf

IMTAKT Publication

Development of a Novel Amino Acids Analysis Column for LC-MS without Derivatization

BIOINDUSTRY (August,2014)

LC-MS

Actual Sample Data

Applications for Actual Samples

Applications NEW

User Articles

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Nitrogen dynamics and fertilisation use efficiency: carry-over effect of crop limitation


Biomarker profiling of postmortem blood for diabetes mellitus and discussion of possible applications of metabolomics for forensic casework


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Rice ACID PHOSPHATASE 1 regulates Pi stress adaptation by maintaining intracellular Pi homeostasis


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Plasma kynurenine to tryptophan ratio is not associated with undernutrition in adults but reduced after nutrition intervention: results from a community-based study


Characterization of Organic Solvent Extracts and Protein Extracted From Omani Seaweed “Hypnea bryoides”


VPS34-dependent control of apical membrane function of proximal tubule cells and nutrient recovery by the kidney


Comprehensive targeted metabolomic study in the lung, plasma, and urine of PPE/LPS-induced COPD mice model


Evaluation of fructo-, inulin-, and galacto-oligosaccharides on the Maillard reaction products in model systems with whey protein


Quantitative determination of 2-oxo-imidazole-containing dipeptides by high-performance liquid chromatography/tandem mass spectrometry


Climate-driven changes to taste and aroma determining metabolites in an economically valuable portunid (Portunus armatus) have implications for future harvesting


Studies on the reaction of dietary methylglyoxal and creatine during simulated gastrointestinal digestion and in human volunteers


BIOM-26. Genome-wide somatic copy number alteration signatures have prognostic implications for adult astrocytic glioma


BIOM-24. Targeted next-generation sequencing (NGS) of temporally matched cerebrospinal fluid (CSF) and tumor tissue in adult glioma


Natural polyphenols convert proteins into histone-binding ligands


2-Oxo-imidazole dipeptides in meat


Facilitation of auxin biosynthesis and metabolism by salt stress in rice plants


Carryover effects of crop thinning and foliar N fertilisation on grape amino N composition


Effect of yeast assimilable nitrogen content on fermentation kinetics, wine chemical composition and sensory character in the production of assyrtiko wines


Inhibition of glutaminase 1-mediated glutaminolysis improves pathological cardiac remodeling


Phenotypic differences between Edwardsiella piscicida and Edwardsiella anguillarum isolates in Japan


Cinnamomum verum J. Presl Bark Contains High Contents of Nicotinamide Mononucleotide


Bimonthly variation in nutrient composition and taste components of Crassostrea gigas cultured in Rushan, Southern yellow sea


Phytochemical profiles of Citrus hybrid kanpei and Citrus reticulata natsumi: Analysis of volatile and non-volatile components


Influence of lactose on the Maillard reaction and dehydroalanine-mediated protein cross-linking in casein and whey


Investigation of β-Substitution Activity of O-Acetylserine Sulfhydrolase from Citrullus vulgaris


Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter


BrnQ-type branched-chain amino acid transporters influence Bacillus anthracis growth and virulence


Plasma amino acids and risk of impaired lower-extremity function and role of dietary intake: a nested case-control study in older adults


Determination of amino acids in human biological fuids by high-performance liquid chromatography: critical review


Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats


Glucose metabolism and pyruvate carboxylase enhance glutathione synthesis and restrict oxidative stress in pancreatic islets


Effects of induction on the farrowing process and piglet blood parameters at the time of farrowing


Nasopharyngeal metabolomics and machine learning approach for the diagnosis of influenza


Peripheral Hybrid CB1R and iNOS Antagonist MRI-1867 Displays AntiFibrotic Efficacy in BleomycinInduced Skin Fibrosis


Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxication


Changes in sow milk lipidome across lactation occur in fatty acyl residues of triacylglycerol and phosphatidylglycerol lipids, but not in plasma membrane phospholipids


Nutritional evaluation of taurine and methionine in commercially available cat foods


Combining ion chromatography with mass spectrometry and inductively coupled plasma-mass spectrometry: Annual review 2020


2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides


High-Precision Sulfur Metabolomics Innovated by a New Specific Probe for Trapping Reactive Sulfur Species


Changes in sow milk lipidome across lactation occur in fatty acyl residues of triacylglycerol and phosphatidylglycerol lipids, but not in plasma membrane phospholipids


Hydroxyurea improves nitric oxide bioavailability in humanized sickle cell mice


Arid5a Promotes Immune Evasion by Augmenting Tryptophan Metabolism and Chemokine Expression


Comparisons of Chemical Composition, Flavor and Bioactive Substances between Korean and Imported Velvet Antler Extracts


α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube


Dynamin-related protein 1 inhibition reduces hepatic PCSK9 secretion


Fermented milk retains beneficial effects on skeletal muscle protein anabolism after processing by centrifugation and supernatant removal


Beneficial effects of the consumption of sun-dried radishes (<i>Raphanus sativus</i> cv. YR-Hyuga-Risou) on dyslipidemia in apolipoprotein E-deficient mice


Relationships among the Hexosamine Biosynthetic Pathway, Sleep Disturbance, and Cognition in Obstructive Sleep Apnea


Effects of suppressing protein structural changes on the excellent gelling properties of dried egg white via dry-heat treatment


Relative Late Gestational Muscle and Adipose Thickness Reflect the Amount of Mobilization of These Tissues in Periparturient Dairy Cattle


Distribution and quantitative analysis of homoanserine and its 2-oxo derivative in mouse tissues.


Mammary Development in Gilts at One Week Postnatal Is Related to Plasma Lysine Concentration at 24 h after Birth, but Not Colostrum Dose


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Increased solubility and functional properties of precipitated Alfalfa protein concentrate subjected to pH shift processes


Influence of Excessively High Temperatures on the Fruit Growth and Physicochemical Properties of Shiranuhi Mandarin in Plastic-Film Greenhouse Cultivation


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Flavor and Metabolite Profiles of Meat, Meat Substitutes, and Traditional Plant-Based High-Protein Food Products Available in Australia


Ulva fenestrata protein - Comparison of three extraction methods with respect to protein yield and protein quality


Homogenization in water improves the recovery rate of imidazole dipeptides in meat


Preparation of High GABA-Enriched Yeast Extract by Non-Saccharomyces Yeasts Isolated from Korean Traditional Fermented Soybean Product


Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats


Characterization of Chinese hamster ovary cell culture feed media precipitate


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DEVELOPMENT AND VALIDATION OF AN LC-MS/MS ASSAY TO MEASURE GLUTATHIONE, GLUTATHIONE DISULFIDE, CYSTEINE, AND CYSTINE IN HUMAN BRAIN AND HUMAN GLIOBLASTOMA


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Quantification of bovine plasma amino acids via liquid chromatography-electrospray ionizationmass spectrometry: Comparison of underivatized and precolumn derivatized methods


Circulating amino acids as fingerprints of visceral adipose tissue independent of insulin resistance: a targeted metabolomic research in women


Nitrogen Balance and Bioavailability of Amino Acids in Spirulina Diet-Fed Wistar Rats


Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis


Amino Acid Signature of Oxidative Stress in Patients with Type 2 Diabetes: Targeted Exploratory Metabolomic Research


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Aloe attenuates acute colonic inflammation in mice and enhances tight junction proteins in intestinal epithelial cells


Analysis of Amino acid Contents in Food for Revision of The Korean Food Composition Table


Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats


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Control of protein function through oxidation and reduction of persulfidated states


Impact of crop load on nitrogen uptake and reserve mobilisation in Vitis vinifera


Resistance exercise with anti-inflammatory foods attenuates skeletal muscle atrophy induced by chronic inflammation


Disulfide bond formation is not crucial for the heat-induced interaction between β-lactoglobulin and milk fat globule membrane proteins


Glucose-dependent partitioning of arginine to the urea cycle protects β-cells from inflammation


A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells


Effect of half-dry processing on umami components of vegetables and mushrooms


Effects of dietary intake of heat-inactivated Lactobacillus gasseri CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model


Effects of administration of ascorbic acid and low-dose hydrocortisone after infusion of sublethal doses of lipopolysaccharide to horses


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Free advanced glycation end product distribution in blood components and the effect of genetic polymorphisms


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Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination


Carnosine and anserine in chicken can quench toxic acrylamide under cooking conditions: Mass spectrometric studies on adduct formation and characterization


Suppressed Methionine γ-Lyase Expression Causes Hyperaccumulation of S-Methylmethionine in Soybean Seeds


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Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin


MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis


Volatile and non-volatile metabolite changes in 140-day stored vacuum packaged chilled beef and potential shelf life markers


The branched chain aminotransferase IlvE promotes growth, stress resistance and pathogenesis of Listeria monocytogenes


Impacts on product quality attributes of monoclonal antibodies produced in CHO cell bioreactor cultures during intentional mycoplasma contamination events


Effects of Lanthionine and Lysinoalanine on Heat-induced Gelation of Egg White


B38-CAP is a bacteria-derived ACE2-like enzyme that suppresses hypertension and cardiac dysfunction


Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease


β-Aminoisobutyric Acid Suppresses Atherosclerosis in Apolipoprotein E-Knockout Mice


Comparison of Tastes-Related Components and Eating Quality between Hanwoo Steer and Cow Longissimus thoracis Muscles


Novel method for measuring aquatic bacterial productivity using D10-leucine based on protein synthesis rate


Plasma amino acid levels in a cohort of patients in Turkey with classical phenylketonuria


Kynurenic acid is a potential overlapped biomarker between diagnosis and treatment response for depression from metabolome analysis


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An accurate and sensitive analytical method for the simultaneous determination of glycine, methionine and homocysteine in biological matrices by matrix matching strategy and LC-quadrupole-time-of-flight-MS/MS


Integration of Time-Series Transcriptomic Data with Genome-Scale CHO Metabolic Models for mAb Engineering


Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics


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Severity of the autoimmune encephalomyelitis symptoms in mouse model by inhibition of LAT‑1 transporters


Development and application of a multiple reaction monitoring mass spectrometry method for absolute quantification of lysinoalanine and lanthionine in dairy products


Enhancement of amino acid contents by germination in a Korean weedy rice germplasm


Metabolic profiling by reversed-phase/ion-exchange mass spectrometry


Absolute quantitative analysis of intact and oxidized amino acids by LC-MS without prior derivatization


A reliable LC-MS/MS method for targeted analysis of amino acid metabolome: Method validation and application to studies on amino acid dynamics during tumor progression


Plasma amino acid levels in a cohort of patients in Turkey with classical phenylketonuria


Effect of half-dry processing on umami components of vegetables and mushrooms


Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)


A Paleolithic diet lowers resistant starch intake but does not affect serum trimethylamine-N-oxide concentrations in healthy women


Association Between Serum β-Alanine and Risk of Dementia: The Hisayama Study


The Absence of Pyruvate Kinase Affects Glucose-Dependent Carbon Catabolite Repression in Bacillus subtilis


LC-ESI-MS/MS quantification of carnosine, anserine, and balenine in meat samples


Downregulation of aspartoacylase during the progression of myelin breakdown in the dmy mutant rat with mitochondrial magnesium channel MRS2 defect


Volatile Flavor Components as a Function of Electrical Stimulation and Chiller Aging for m. longissimus and biceps femoris of Hanwoo Beef


Bioinformatic and Functional Evaluation of Actinobacterial Piperazate Metabolism


Impacts of intentional mycoplasma contamination on CHO cell bioreactor cultures


Metabolomics Based on MS in Mice with Diet-Induced Obesity and Type 2 Diabetes Mellitus: the Effect of Vildagliptin, Metformin, and Their Combination


Increase of Arginine Dimethylation Correlates with the Progression and Prognosis of ALS


Consumer Perception of Snack Sausages Enriched with Umami-Tasting Meat Protein Hydrolysates


Determination of Glycine in Body Fluids at Trace Levels Using the Combination of Quadrupole Isotope Dilution Strategy and Liquid Chromatography-Quadrupole Time of Flight-Tandem Mass Spectrometry


Use of mTRAQ Derivatization Reagents on Tissues for Imaging Neurotransmitters by MALDI Imaging Mass Spectrometry: the Triple Spray Method


Growth and Physiological Characteristics in a Halophyte Suaeda glauca under Different NaCl Concentrations


Dosing Time-Dependent Changes in the Anti-tumor Effect of xCT Inhibitor Erastin in Human Breast Cancer Xenograft Mice


Real-time Quantification and Supplementation of Bioreactor Amino Acids to Prolong Culture Time and Maintain Antibody Product Quality


Analytical Strategies for the Determination of Amino Acids: Past, Present and Future Trends


The Performance Investigation of Bimodal Cation-Exchange/Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry by Modifying Mobile Phase Composition in Amino Acid Separation


Measuring Biomarkers in Blood Developing a Robust, High Throughput LC-MS Method


Umami Taste, Free Amino Acid Composition, and Volatile Compounds of Brown Seaweeds


Rapid Quantification of Underivatized Alloisoleucine and Argininosuccinate Using Mixed-Mode Chromatography with Tandem Mass Spectrometry


Development and Validation of a Simple LC-MS/MS Method for the Simultaneous Quantitative Determination of Trimethylamine-N-Oxide and Branched Chain Amino Acids in Human Serum


2-Oxo-histidine-containing dipeptides are functional oxidation products


Ablation of adipocyte creatine transport impairs thermogenesis and causes diet-induced obesity


A reliable LC-MS/MS method for the quantification of natural amino acids in mouse plasma: Method validation and application to a study on amino acid dynamics during hepatocellular carcinoma progression


Soft-tissue evidence for homeothermy and crypsis in a Jurassic ichthyosaur


AGEsの精密分析と食品化学,医学への応用AGEsの精密分析により新しい事実が明らかに


Classification of 1-methylcyclopropene treated apples by fluorescence fingerprint using partial least squares discriminant analysis with stepwise selectivity ratio variable selection method


Influence of saliva on individual in-mouth aroma release from raw cabbage (Brassica oleracea var. capitata f. rubra L.) and links to perception


The Influence of Dietary Habits and Meat Consumption onPlasma 3-Methylhistidine—A Potential Marker for MuscleProtein Turnover


NNMT activation can contribute to the development of fatty liver disease by modulating the NAD+ metabolism


The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content


Direct Quantitation of Amino Acids in Human Serum Using a Stepwise-Dilution Strategy and a Mixed-Mode Liquid Chromatography-Tandem Mass Spectrometry Method


Development of a UPLC-MS/MS Method for the Therapeutic Monitoring of L-Asparaginase


Changes in the quality of beef while dry-aging with Mucor flavus


Changes in free amino acids and hardness in round of Okinawan delivered cow beef during dry- and wet-aging processes


Positional stable isotope tracer analysis reveals carbon routes during ammonia metabolism of Aedes aegypti mosquitoes


The quest for umami: Can sous vide contribute?


Influence of Probiotics-Friendly Pig Production on Meat Quality and Physicochemical Characteristics


Probing Protein Glycation by Chromatography and Mass Spectrometry: Analysis of Glycation Adducts


Elephant's breast milk contains large amounts of glucosamine


Flavour of fermentedfish, insect, game, and pea sauces: Garum revisited


Effects of the pH and Concentration on the Stability of Standard Solutions of Proteinogenic Amino Acid Mixtures


Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection


Direct Quantitation of Amino Acids in Human Serum Using a Stepwise-Dilution Strategy and a Mixed-Mode Liquid Chromatography-Tandem Mass Spectrometry Method


Identification of Polyphenol-Specific Innate Epitopes That Originated from a Resveratrol Analogue


Effects of the 5-HT6 Receptor Antagonist Idalopirdine on Extracellular Levels of Monoamines, Glutamate and Acetylcholine in the Rat Medial Prefrontal Cortex


Development of a Mixed-Mode Chromatography with Tandem Mass Spectrometry Method for the Quantitative Analysis of 23 Underivatized Amino Acids in Human Serum


High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report


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「おきなわブランド」ドライエイジングビーフ生産技術の確立(1)熟成湿度と衛生指標、歩留り、アミノ酸含量との関係


Simultaneous Quantitation of Advanced Glycation End Products in Soy Sauce and Beer by Liquid Chromatography-Tandem Mass Spectrometry without Ion-Pair Reagents and Derivatization


Hybrid Inhibitor of Peripheral Cannabinoid-1 Receptors and Inducible Nitric Oxide Synthase Mitigates Liver Fibrosis


Effect of Natural Betaine on Estimates of Semen Quality in Mature AI Boars during Summer Heat Stress


High Throughput Sequencing of Cyclic Peptide Immobilized on a Gel-Type Single Bead


H+/Peptide Transporter (PEPT2) is Expressed in Human Epidermal Keratinocytes and is Involved in Skin Oligopeptide Transport


Chemical and Physical Properties of Thai Traditional Shrimp Paste (Ka-pi)


Amino Acid Metabolomics Using LC-MS/MS: Assessment of Cancer-Cell Resistance in a Simulated Tumor Microenvironment


Simultaneous Determination of the Neurotransmitters and Free Amino Acids in Rat Organs by LC-MS Analysis


High-resolution Mass Spectrometry for Detecting Acetylcholine in Arabidopsis


Free Amino Acids Analysis by Liquid Chromatography with Tandem Mass Spectrometry in Several Botanicals with Antioxidant Character


Stable Isotope Labeling of Glycoprotein Expressed in Silkworms Using Immunoglobulin G as a Test Molecule


穀類中の遊離アスパラギンの含有実態について