Wide-Pore Reversed-Phase Column for Bio-polymers
FEATURES
Fixed Phase for High Molecular Weight Reversed-Phase Separation Using 30nm Pores
High separation performance with 3μm silica particles
Moderate surface polarity due to newly developed reversed-phase ligands
Suitable for biomolecules such as proteins up to several hundred thousand Daltons
Enables easier elution of hydrophobic substances compared to C4 wide-pore columns
Technical Information NEW
- ●250x4.6mmDAABD-proteins from breast cancer cells
- ●250x4.6mmCollagen from pig skin
- ●250x4.6mmBovine serum
- ●250x3mmBovine milk
PROTEINS
- ●150x4.6mmStandard Proteins
- ●250x4.6mmThyroglobulin (670kDa)
- ●250x4.6mmβ-Glucosidase (183kDa)
- ●250x4.6mmLactferrin (93kDa)
- ●250x4.6mmHemoglobin (64kDa)
- ●250x4.6mmInterferon (20kDa)
- ●250x2mm, 30x2mmInterferon (20kDa)
- ●100x4.6mmAmyloid-beta peptide (4kDa)
- ●250x4.6mmPhycocyanin from Spirulina
OTHERS
User Reports
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●Mallinckrodt, USA
Isopropanol Mobile Phase Beneficial for Reversed-phase Analysis of Peptide Drugs
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●MedImmune, USA
Reversed-phase Analysis of Hydrophobic Proteins
User Articles
Methylglyoxal and D-lactate in cisplatin-induced acute kidney injury: Investigation of the potential mechanism via fluorogenic derivatization liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) proteomic analysis
Shih-Ming Chen, Tsung-Hui Chen, Hui-Ting Chang, Tzu-Yao Lin, Chia-Yu Lin, Pei-Yun Tsai, Kazuhiro Imai, Chien-Ming Chen, Jen-Ai Lee
PLoS ONE, Vol.15 Issue 7, p1-19, 7/10/2020
Immobilized metal affinity chromatography optimization for poly-histidine tagged proteins
Valeria Rigueroa, Robert Clifford, Michael Dawley, Matthew Dickson, Benjamin Gastfriend, Christopher Thompson, Sheau-Chiann Wang, Ellen O’Connor
Journal of Chromatography A, 1629 (2020) 461505
A novel preventive mechanism of gentamicin‐induced nephrotoxicity by atorvastatin
Mei-Chun Lee, Kuei-Ju Cheng, Shih-Ming Chen, Yi-Chieh Li, Kazuhiro Imai, Chun-Ming Lee, Jen-Ai Lee
Biomedical Chromatography, 33, 11, e4639, 2019
Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage
Kaname Ohyama, YukaI keshita, Yuki Fuchigami, Shigeru Kawakami, Mihoko N. Nakashima, Mikiro Nakashima
Journal of Neuroscience Methods, 315, 1-5, 2019
Comparative proteomic analysis identifies exosomal Eps8 protein as a potential metastatic biomarker for pancreatic cancer
Keiichi Ohshima, Keiichi Hatakeyama, Kaori Kanto, Tomomi Ide, Yuko Watanabe, Sachi Moromizato, Kanako Wakabayashi-Nakao, Naoki Sakura, Ken Yamaguchi, Tohru Mochizuki
Oncology Reports, 41, 2, 1019-1034, 2019
Separation Analysis of Adiponectin Multimers by High-performance Liquid Chromatography
Kyosuke NAGASHIMA, Hiroaki MINAMISAWA, Yasuhito NOBUSHI, Tatsuro NAKAGAMA, Kazunori SAITOH and Hiromichi ASAMOTO
Bulletin of the Society of Sea Water Science, Japan, 71, 354-360, 2017
Rapid, Sensitive and Simple LC-MS/MS Method Development and Validation for Estimation of Phenytoin in Human Plasma
RAMPALLI SHANKAR SHESHU and PALANI SHANMUGASUNDARAM
Asian Journal of Chemistry; 29, 10 , 2305-2310, 2017
Monitoring Quality of Critical Reagents Used in Ligand Binding Assays with Liquid Chromatography Mass Spectrometry (LC-MS)
Brian Geist, Adrienne Clements-Egan andTong-Yuan Yang
Protein Analysis using Mass Spectrometry: Accelerating Protein Biotherapeutics from Lab to Patient, John Wiley & Sons, 2017
Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε)
Yoko Yamauchi, Hikari Kimoto, Xianyu Yang, Sergey Filkin, Yuri Utkin, Tai Kubo & Hidetoshi Inagaki
Bioscience, Biotechnology, and Biochemistry, 80, 1, 158-161, 2016
Development and Application of FD-LC-MS/MS Proteomics Analysis Revealing Protein Expression and Biochemical Events in Tissues and Cells
Tomoko Ichibangase, Kazuhiro Imai
YAKUGAKU ZASSHI, 135, 2, 197-203, 2015
Application of DBS Methodology for a Comparative Assessment of Phenytoin in DBS and Plasma Samples of a Pharmacokinetic Rat Study Using LC-MS/MS Method
Vijay Kumar Raina, Wishu Shrivastava, Akena Venkatesham, Yuan Song, P.K. Patel
INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH (IJSR ), 4, 5, 206-213, 2015
Nonclinical pharmacokinetics and activity of etirinotecan pegol (NKTR-102), a long-acting topoisomerase 1 inhibitor, in multiple cancer models
Ute Hoch, Carl-Michael Staschen, Randall K. Johnson, Michael A. Eldon
Cancer Chemotherapy and Pharmacology, 74, 6, 1125-1137, 2014
Straightforward proteomic analysis reveals real dynamics of proteins in cells
Tomoko Ichibangase, Kazuhiro Imai
Journal of Pharmaceutical and Biomedical Analysis, 101, 31-39, December 2014
Increased Concentrations of Apo A-I and Apo A-II Fragments in the Serum of Patients With Hepatocellular Carcinoma by Magnetic Beads--Assisted MALDI-TOF Mass Spectrometry
Yang Liu, Kazuyuki Sogawa, Masahiko Sunaga, Hiroshi Umemura, Mamoru Satoh, Takahiro Kazami, Masaharu Yoshikawa, Takeshi Tomonaga, Osamu Yokosuka, Fumio Nomura
American Journal of Clinical Pathology, 141, 1, 52-61, Jan 2014
A novel approach for the simultaneous quantification of a therapeutic monoclonal antibody in serum produced from two distinct host cell lines
Brian J. Geist, Darryl Davis, Thomas McIntosh, Tong-Yuan Yang, Kenneth Goldberg, Chao Han, Charles Pendley and Hugh M. Davis
mAbs, 5, 1, 150-161, 2013
Efficient chromatographic separation of intact proteins derivatized with a fluorogenic reagent for proteomics analysis
Tomoko Ichibangase, Itaru Yazawa and Kazuhiro Imai
Biomedical Chromatography, 27, 11, 1520-1523, 2013
Proteome analysis of altered proteins in streptozotocin-induced diabetic rat kidney using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry method
Pei-Yun Tsai, Shih-Ming Chen, Hsiang-Yin Chen, Yi-Chieh Li, Kazuhiro Imai, Kuang-Yang Hsu, Jen-Ai Lee
Biomedical Chromatography, 27, 3, 382-389, 2013
An FD-LC-MS/MS Proteomic Strategy for Revealing Cellular Protein Networks: A Conditional Superoxide Dismutase 1 Knockout Cells
Tomoko Ichibangase, Yasuhiro Sugawara, Akio Yamabe, Akiyo Koshiyama, Akari Yoshimura, Takemi Enomoto, Kazuhiro Imai
PloS one, 7(9), e45483, 2012
The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma
Asako Kimura, Kazuyuki Sogawa, Mamoru Satoh, Yoshio Kodera, Osamu Yokosuka, Takeshi Tomonaga, and Fumio Nomura
International Journal of Proteomics, Volume 2012, Article ID 623190, 2012
Identification of a Novel Biomarker for Biliary Tract Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Shintaro Kikkawa, Kazuyuki Sogawa, Mamoru Satoh, Hiroshi Umemura, Yoshio Kodera, Kazuyuki Matsushita, Takeshi Tomonaga, Masaru Miyazaki, Osamu Yokosuka, and Fumio Nomura
International Journal of Proteomics, Volume 2012, Article ID 108609, 2012
Alkylation of cysteine 468 in Stat3 defines a novel site for therapeutic development
Ralf Buettner, Renzo Corzano, Rumana Rashid, Jianping Lin, Maheswari Senthil, Michael Hedvat, Anne Schroeder, Allen Mao, Andreas Herrmann, John Yim, Hongzhi Li, Yate-Ching Yuan, Kenichi Yakushijin, Fumiko Yakushijin, Nagarajan Vaidehi, Roger Moore, Gabriel Gugiu, Terry D. Lee, Richard Yip, Yuan Chen, Richard Jove, David Horne, and John C. Williams
ACS Chemical Biology, 6, 5, 432-443, 2011
Proteomic profiling of k-11706 responsive proteins
Horie M, Kawashima Y, Naka A, Matsumoto K, Kodera Y, Maeda T, Iida K
International Journal of Sports Medicine, 32(7), 559-564, 2011
14-3-3 Protein beta/alpha as a urinary biomarker for renal cell carcinoma: proteomic analysis of cyst fluid
Satoru Minamida, Masatsugu Iwamura, Yoshio Kodera, Yusuke Kawashima, Kenichi Tabata, Kazumasa Matsumoto, Tetsuo Fujita, Takefumi Satoh, Tadakazu Maeda, Shiro Baba
Analytical and Bioanalytical Chemistry, 401, 1, 245-252, July 2011
Identification of vitronectin as a novel serum marker for early breast cancer detection using a new proteomic approach
Masami Kadowaki, Takafumi Sangai, Takeshi Nagashima, Masahiro Sakakibara, Hideyuki Yoshitomi, Shigetsugu Takano, Kazuyuki Sogawa, Hiroshi Umemura, Koya Fushimi, Yukio Nakatani, Fumio Nomura, Masaru Miyazaki
Journal of Cancer Research and Clinical Oncology, 137, 7, 1105-1115, July 2011
Liquid chromatographic separation of proteins derivatized with a fluorogenic reagent at cysteinyl residues on a non-porous column for differential proteomics analysis
Akiyo Koshiyama, Tomoko Ichibangase, Kyoji Moriya, Kazuhiko Koike, Itaru Yazawa, Kazuhiro Imai
Journal of Chromatography A, 1218, 22, 3, 3447-3452, 2011
Towards clinical proteomics analysis
Kazuhiro Imai, Akiyo Koshiyama, Katsunori Nakata
Biomedical Chromatography, 25, 1-2, 59-64, 2011
Increased Serum Levels of Pigment Epithelium-Derived Factor by Excessive Alcohol Consumption—Detection and Identification by a Three-Step Serum Proteome Analysis
Kazuyuki Sogawa, Yoshio Kodera, Mamoru Satoh, Yusuke Kawashima, Hiroshi Umemura, Katsuya Maruyama, Hirotaka Takizawa, Osamu Yokosuka, Fumio Nomura
Alcoholism, Clinical and Experimental Research, 35, 2, 211-217, 2011
Proteomic Study of Sera from Patients with Bladder Cancer: Usefulness of S100A8 and S100A9 Proteins
SHO MINAMI, YUICHI SATO, TOSHIHIDE MATSUMOTO, TAIHEI KAGEYAMA, YUSUKE KAWASHIMA, KODERA YOSHIO, JUN-ICHIRO ISHII, KAZUMASA MATSUMOTO, RYO NAGASHIO and ISAO OKAYASU
Cancer Genomics and Proteomics, 7, 4, 181-189, 2010
A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity
Kaname Ohyama, Mari Tomonari, Tomoko Ichibangase, Hideto To, Naoya Kishikawa, Kenichiro Nakashima, Kazuhiro Imai, Naotaka Kuroda
Biochemical Pharmacology, 80, 4, 540-547, 2010
Stomatal Density is Controlled by a Mesophyll-Derived Signaling Molecule
Tatsuhiko Kondo, Ryoko Kajita, Aya Miyazaki, Mayumi Hokoyama, Touko Nakamura-Miura, Satoko Mizuno, Yuichi Masuda, Kazuhiro Irie, Yuki Tanaka, Shinobu Takada, Tatsuo Kakimoto, and Youji Sakagami
Plant & Cell Physiology, 51, 1, 1-8, 2010
Synthesis and evaluation of a fluorogenic reagent for proteomic studies: 7-fluoro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide (DAABD-F)
Akiyo Koshiyamaa and Kazuhiro Imai
Analyst, 135, 2119-2124, 2010
YKL-40 IDENTIFIED BY PROTEOMIC ANALYSIS AS A BIOMARKER OF SEPSIS
Noriyuki Hattori, Shigeto Oda, Tomohito Sadahiro, Masataka Nakamura, Ryuzo Abe, Koichiro Shinozaki, Fumio Nomura, Takeshi Tomonaga, Kazuyuki Matsushita, Yoshio Kodera, Kazuyuki Sogawa, Mamoru Satoh, and Hiroyuki Hirasawa
SHOCK, 32, 4, 393-400, 2009
A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins
Yusuke Kawashima, Tomoyuki Fukuno, Mamoru Satoh, Hiroki Takahashi, Takashi Matsui, Tadakazu Maeda, Yoshio Kodera
Journal of Electrophoresis, 53, 1, 13-18, 2009
Limitation of immunoaffinity column for the removal of abundant proteins from plasma in quantitative plasma proteomics
Tomoko Ichibangase, Kyoji Moriya, Kazuhiko Koike and Kazuhiro Imai
Biomedical Chromatography, 23, 5, 480-487, May 2009
Application of Fluorogenic Derivatization-Liquid Chromatography-Tandem Mass Spectrometric Proteome Method to Skeletal Muscle Proteins in Fast Thoroughbred Horses
Tomoko Ichibangase and Kazuhiro Imai
Journal of Proteome Research, 8, 4, 2129-2134, 2009
Application of an improved proteomics method, fluorogenic derivatization-liquid chromatography-tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain
Hiromichi Asamoto, Tomoko Ichibangase, Kazuo Uchikura, Kazuhiro Imai
Journal of Chromatography A, 1208, 1-2, 147-155, October 2008
Proteomics of Caenorhabditis elegans over-expressing human alpha-synuclein analyzed by fluorogenic derivatization-liquid chromatography/tandem mass spectrometry: identification of actin and several ribosomal proteins as negative markers at early Parkinson's disease stages
Tomoko Ichibangase, Hiroshi Saimaru, Norio Takamura, Tomoki Kuwahara, Akihiko Koyama, Takeshi Iwatsubo, Kazuhiro Imai
Biomedical Chromatography, 22, 3, 232-234, 2008
Differential proteome analysis for identifying the age-related proteins in three mouse brain regions during the developmental stage by fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) method
H. Asamoto, T. Ichibangase, K. Uchikura and K. Imai
Luminescence 23, 195, 2008
A Proteomics Method Revealing Disease-Related Proteins in Livers of Hepatitis-Infected Mouse Model
Tomoko Ichibangase, Kyoji Moriya, Kazuhiko Koike, and Kazuhiro Imai
Journal of Proteome Research, 6, 7, 2841-2849, 2007
A new fluorogenic reagent aimed at simultaneous determination of peptides or proteins in two different samples
Hiroshi Saimaru, Mayumi Masuda, Chifuyu Toriumi, Tomofumi Santa, Eiko Ichikawa, Norio Takamura and Kazuhiro Imai
Journal of Materials Chemistry, 15, 2865-2872, 2005