Uses volatile phases for size exclusion chromatography
Separating biomolecules, and synthetic polymers via GPC
FEATURES
Unified Size Exclusion Chromatography (SEC) (GFC + GPC)
Accommodates hydrophilic polymers such as proteins (GFC)
Supports synthetic polymers like polystyrene (GPC)
Compatible with volatile mobile phases for LC-MS analysis
MAIN SPECIFICATIONS
| Base material: | Fully porous high-purity silica gel |
| Particle size: | 3μm |
| Pore size: | 30nm |
| Ligand: | Diol group |
| Target molecular weight: | Below 1MDa |
| pH range: | pH 1-8 |
| Maximum pressure: | 300bar / 4,300psi / 30MPa |
| USP Code: | L20, L33, L59 |
Challenges of Traditional Size Exclusion Chromatography (SEC) Columns
- SEC columns with diol groups on silica have required harsh mobile phases for LC systems, such as 100 mM high-concentration phosphate buffer + 300 mM NaCl.
- In GPC mode using organic polymer substrates, there have been difficulties in mobile phase substitution due to swelling and contraction of packing materials.
Advantages of this Product
Intrada SEC column overcomes these challenges by developing a new chemical modification method for diol groups on the surface of fully porous silica gel substrates. This next-generation SEC column combines the mechanical strength of silica-based packing materials with the chemical durability of polymer-based packing materials.
It offers versatility to handle hydrophilic biomolecules like proteins, polysaccharides, and nucleic acids, as well as non-aqueous SEC for polymers like polystyrene. Moreover, it supports the use of volatile mobile phases such as 100 mM ammonium formate, making it suitable not only for traditional RI and UV detection but also for new analytical methods using evaporative light scattering detectors (ELSD) and mass spectrometers (MS).
SEC-MS Applications
-Precise Molecular Weight Measurement of Purified Proteins (SEC-MS)-
- LC-TOF/MS Analysis of PeroxidaseNEW
- LC-TOF/MS Analysis of Thermolysin
- LC-TOF/MS Analysis of α-Amylase
- LC-TOF/MS Analysis of Concanavalin A
- LC-TOF/MS Analysis of Insulin (Human, recombinant)
- LC-TOF/MS Analysis of Insulin (Bovine Pancreas)
- LC-TOF/MS Analysis of Myoglobin
- LC-TOF/MS Analysis of Deoxyribonuclease I (DNase I)
- LC-TOF/MS Analysis of Ribonuclease A
- LC-TOF/MS Analysis of Lysozyme
- LC-TOF/MS Analysis of Lactoferrin
- LC-TOF/MS Analysis of Transferrin (apo)
- LC-TOF/MS Analysis of Transferrin (holo)
- LC-TOF/MS Analysis of Casein (Bovine milk)
- LC-TOF/MS Analysis of Recombinant human serum albumin (rHSA)
- LC-TOF/MS Analysis of Alpha-Lactalbumin
- LC-TOF/MS Analysis of Cytochrome c (cyt c)
- LC-TOF/MS Analysis of Hemoglobin
- LC-TOF/MS Analysis of Ovalbumin
- LC-TOF/MS Analysis of Bovine Serum Albumin (BSA)
- LC-Orbitrap MS Analysis of α-Amylase
- LC-Orbitrap MS Analysis of Elastase
- LC-Orbitrap MS Analysis of Recombinant (genetically modified protein) Human Serum Albumin (rHSA)
Technical Information
Applications NEW
Peptides and proteins
- ● Monoclonal Antibody (Anti-GFP, green fluorescent protein, Rat IgG2a)
- ● Monoclonal Antibody (Anti-GST, Mouse IgG2a-k)
- ● Monoclonal Antibody (Anti Derf II)
- ● Monoclonal Antibody (Anti Human luteinizing hormone)
- ● Monoclonal Antibody (Anti Hexanoyl-lysine)
- ● Monoclonal Antibody (Anti alpha-Fetoprotein)
- ● Monoclonal Antibody (mAb)
- ● IgG in Human Serum
- ● Human Serum
- ● LC-TOF/MS Analysis of Peroxidase MS NEW
- ● LC-TOF/MS Analysis of Thermolysin MS
- ● LC-TOF/MS Analysis of α-Amylase MS
- ● Concanavalin A MS
- ● Insulin (Bovine Pancreas) MS
- ● Insulin (Bovine Pancreas) MS
- ● Myoglobin MS
- ● Deoxyribonuclease I (DNase I) MS
- ● Ribonuclease A MS
- ● Lysozyme MS
- ● Lactoferrin MS
- ● Transferrin (apo) MS
- ● Transferrin (holo) MS
- ● Casein (Bovine milk) MS
- ● Recombinant human serum albumin (rHSA) MS
- ● LC-TOF/MS Analysis of Alpha-Lactalbumin MS
- ● LC-TOF/MS Analysis of Cytochrome c (cyt c) MS
- ● Ovalbumin MS
- ● LC-TOF/MS Analysis of Bovine Serum Albumin (BSA) MS
- ● LC-Orbitrap MS Analysis of α-AmylaseMS(Provided by Dr. Kuwata, Nagoya University) MS
- ● LC-Orbitrap MS Analysis of ElastaseMS(Provided by Dr. Kuwata, Nagoya University) MS
- ● LC-Orbitrap MS Analysis of Recombinant Human Serum Albumin (rHSA)MS(Provided by Dr. Kuwata, Nagoya University) MS
- ● Phycocyanin (Derived from Spirulina)
- ● Comparison of Brewer's Yeast Tablets
- ● Ribonuclease A (RNaseA)
- ● Defatted Soy Extract
- ● BSA (Bovine Serum Albumin) Monomer and Dimer
- ● Hemoglobin
- ● α-Amylase
- ● β-Glucosidase
- ● Xanthine Oxidase
- ● Chymotrypsinogen A
- ● Casein
- ● Lactoferrin
- ● Lactalbumin
- ● BSA
- ● Myoglobin
- ● Ovoalbumin
- ● Polyglutamic Acid
- ● Lectin (Concanavalin A)
- ● Insulin
- ● Collagen Peptide
- ● Polylysine
Biomacromolecules
- ● Arabinogalactan (from Larch Wood) MS
- ● Sodium alginate 500-600 cP
- ● Isomalto-Dextrin
- ● Lignin
- ● Mucin
- ● Xanthan Gum
- ● Glycogen
- ● Lipopolysaccharide (E.coli O127)
- ● Hyaluronic Acid
- ● Carrageenan
- ● Dextran
- ● Inulin
- ● Fucoidan
- ● Mannan
- ● Pectin
- ● Proteoglycan
- ● Mucopolysaccharides (Glycosaminoglycans, GAGs)
- ● Pullulan <RID>
- ● DNA ladder (20bp) <UV>
Synthetic polymers
- ● Anionic Surfactant (LAS)
- ● Polystyrene Sulfonate
- ● Polyacrylic Acid
- ● Poly(lactic-co-glycolic acid) 50/50 (PLGA)
- ● Poly(lactic-co-glycolic acid) 75/25 (PLGA)
- ● Poly(lactic acid) (5, 10, 15, 20kDa PLA)
- ● Poly(lactic acid) (PLA)
- ● Cellulose Acetate (Acetate Cellulose)
- ● Hydroxypropyl Methylcellulose, Hypromellose (HPMC)
- ● Hydroxypropyl Cellulose (HPC)
- ● Carboxymethyl Cellulose (CMC)
- ● Dextran Sodium Sulfate (DSS)
- ● Polyvinyl Alcohol (PVA)
- ● Polyvinyl Acetate (PVAc)
- ● Polyvinylpyrrolidone (PVP)
- ● Polystyrene
- ● Polyethylene Glycol (PEG)
Other