World’s First High-Resolution 2 μm Non-Porous ODS Column
FEATURES
Reversed-phase separation of biomolecules and synthetic polymers up to several tens of millions Da
Exceptional separation performance for peptides and proteins
Ultra-high theoretical plate column with 2 μm particle size and 250 mm length
Separation characteristics different from porous ODS columns
Shows high separation performance at low flow rates even with standard HPLC
Demonstrates outstanding performance in reversed-phase separation of proteins such as antibodies
Demonstrates outstanding performance in reversed-phase separation of proteins such as antibodies
Using the non-porous ODS column Presto FF-C18, many peaks previously unseen with traditional wide-pore reversed-phase columns are detected for proteins and monoclonal antibodies (mAb). Peaks that appeared broad with conventional columns are actually multiple components. This is likely due to Presto FF-C18 recognizing conformational differences related to S-S bond positions in protein folding, rather than denaturation. Variations in amino acid sequences, impurities, or glycosylation may also contribute, highlighting the column's ability to detect higher-order protein structures.
The detection of many peaks previously unseen with traditional columns using Presto FF-C18 is expected to advance structural analysis for antibody drugs and other protein therapeutics.
The non-porous ODS column Presto FF-C18 excels in reversed-phase separation not only for proteins but also for biomolecules such as polysaccharides and DNA. It is also applicable to synthetic polymers. Sharp, quantifiable peaks that were difficult to achieve with traditional SEC mode can be obtained. The use of detectors suitable for high polymers, such as ELSD, is expected to introduce a new reversed-phase separation method for polymer analysis.
Low Carryover
Presto FF-C18 exhibits excellent low carryover performance in pharmaceutical LC-MS analysis. This is likely due to its nonporous silica, which avoids non-specific adsorption occurring inside pores.
Reference:
Universal LC-MS Method for Minimized Carryover in a Discovery Bioanalytical Setting
John S. Williams, Stephanie H. Donahue, Hong Gao, Christopher L. Brummel
Bioanalysis (2012) 4(9), 1025-1037
https://www.researchgate.net/publication/225051947_Universal_LC-MS_method_for_minimized_carryover_in_a_discovery_bioanalytical_setting
Key Application
Particularly notable key applications using the Presto FF-C18 column are introduced in this section.
Technical Information
- ●For precise separation of peptides and proteins... - Long columns and high temperature with a shallow gradient are effective.
- ●Monoclonal antibodies (mAb) can be separated... - Differences in conformation (isoforms).[Reference: Analytical Methods, 5, 5899-5902, 2013]
- ●Gradient mixers... - Improve separation efficiency by reducing volume.
- ●Since there are no pores... - It can separate DNA molecules as large as several tens of millions Daltons.
- ●In the case of conventional gel filtration (SEC) where separation of difficult polymers is challenging... - Hyaluronic acid and chondroitin sulfate can be separated.
- ●Even synthetic polymers... - Recognition of chain lengths of polystyrene sulfonic acid is possible.
Applications NEW
Peptides and Proteins
- ● Papain MS NEW
- ● Recombinant Human Serum Albumin (rHSA) MS
- ● Lactoferrin MS
- ● Lysozyme MS
- ● Casein MS
- ● Transferrin (apo) MS
- ● Transferrin (holo) MS
- ● human Insulin MS
- ● Myoglobin MS
- ● Alpha-Lactalbumin MS
- ● Cytochrome C (cyt c) MS
- ● Bovine serum albumin (BSA) MS
- ● Phycocyanin from Spirulina
- ● Transferrin
- ● Yeast Tablet Extract
- ● Soy Protein
- ● Recombinant Human Serum Albumin
- ● Recombinant and Biosynthetic Human Serum Albumin (HSA)
- ● Recombinant Human Serum Albumin (rHSA)
- ● Peptide Mapping by LC-FT
- ● Cytochrome c with Formic Acid Mobile Phase
- ● Amyloid β Peptide
- ● Collagen Peptide
- ● Effect of TFA on Peptide Mapping
- ● Peptide Mapping with Formic Acid Mobile Phase
- ● Separation of Various Proteins at 80°C
- ● Separation of Various Proteins at 37°C
- ● Effect of Gradient and Column Length (α-Lactalbumin)
- ● Effect of Gradient and Column Length (Cytochrome c)
- ● Effect of Gradient and Column Length (Thyloglobulin)
- ● Effect of Gradient and Column Length (Lectin)
- ● Effect of Gradient and Column Length (Ovalbumin)
- ● Effect of Gradient and Column Length (BSA)
- ● Effect of Gradient and Column Length (HSA)
- ● Effect of Gradient (Insulin)
- ● Effect of Gradient (Angiotensin)
- ● Effect of Gradient Mixer Volume on Protein Separation
- ● Effect of Column Length on Precise Antibody Separation
- ● Precise Separation of Monoclonal Antibodies (Shallow Gradient)
- ● Effect of Gradient on Monoclonal Antibody Separation
- ● 1200 Peak Separation of Antibodies in Human Serum
- ● Precise Separation of Hemoglobin and Hemocyanin
- ● Non-Porous ODS Superiority in Peptide Mapping
- ● PEGylated Proteins (Effect of Temperature)
- ● PEGylated Proteins (Effect of Column Length)
- ● Proteoglycan
- ● Lectin (Concanavalin A)
- ● Polyamino Acid
- ● Gelatin
- ● Polyglutamic Acid
Nucleic Acid-related
Carbohydrates
- ● Arabinogalactan from Larch Wood
- ● Isomalto-Dextrin
- ● Mucin
- ● Glycogen (Derived from Oyster)
- ● Hyaluronic Acid (Comparison with Wide-Pore Column)
- ● Hyaluronic Acid (Effect of Gradient)
- ● Dextran Sulfate (Difference in Molecular Weight)
- ● Simultaneous Analysis of Hyaluronic Acid and Chondroitin Sulfate
- ● Starch, Amylose, and Amylopectin Derived from Potatoes
- ● Hyaluronic Acid (Effect of Gradient Initial and Final Concentration)
- ● Amylose
- ● Fucoidan
- ● Mannan
- ● Dextran
- ● Mucopolysaccharides (Chondroitin Sulfate, Dermatan Sulfate, Heparin)
- ● Pullulan
- ● Hyaluronic Acid
- ● Alginate
- ● Chondroitin Sulfate, Carrageenan
- ● Lipopolysaccharides Derived from Escherichia coli
- ● Pectin
Synthetic Polymers
- ● Sorbitan fatty acid ester
- ● Span 20 (sorbitan monolaurate)
- ● Polyhexamethylene biguanide, Benzalkonium
- ● Polyoxyethylene Lauryl Ether (Nonionic Surfactant)
- ● Polystyrene Sulfonic Acid
- ● Cellulose Acetate
- ● Polylactic Acid (Lactic/Glycolic Acid Polymer)
- ● Polylactic Acid
- ● Polystyrene (PS)
- ● Polyvinyl Acetate
- ● Polyvinyl Alcohol (PVA)
- ● Polyethylene glycol (PEG) #12000 #20000
- ● Polyethylene Glycol (PEG)
- ● Polyvinylpyrrolidone (PVP)
- ● Hydroxypropyl Methylcellulose (HPMC), Hypromellose (INN)
- ● Hydroxypropyl Methylcellulose (HPMC), Hypromellose (INN)
- ● Hydroxypropyl Cellulose (HPC)
- ● Carboxymethyl Cellulose (CMC)
Others
- ● Lignin
- ● Humic Acid
User Reports
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●Determination of Polymerized Triglycerides by High Pressure Liquid Chromatography and Corona Veo Charged Aerosol Detector
Thermo Fisher Scientific, USA
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●Temperature-Responsive Analysis and HPLC Separation of Vinyl Acetate-Vinyl Alcohol Copolymer
Tokushima University (Graduate School of Technology, Industrial and Social Sciences)
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●Proteome Analysis of Proteins by Fluorescent Derivatization LC-MS/MS Method
Musashino University, Research Institute of Pharmaceutical Sciences
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●Analysis of Chondroitin Sulfate and Carrageenan Using Non-Porous ODS Columns (ELSD)
Perrigo Company of South Carolina Inc., US