From aqueous to non-aqueous phases, high-resolution ODS column
FEATURES
World's highest theoretical plate number of 50,000 plates column (250x4.6mm)
Wide selectivity range from 100% aqueous to non-aqueous mobile phases
Unique end-capping: basic, acid-resistant, alkali-resistant
Wide range of column sizes: inner diameter 0.075 - 28mm / length 10-500mm
High-pressure specifications options
(HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa)
High-pressure metal-free column (MF: 50MPa)
MAIN SPECIFICATIONS
| Particle size: | 3μm (Unison UK-C18) 5μm (Unison US-C18) |
| Base material: | Fully porous high-purity silica gel |
| Pore size: | 13nm |
| Ligand: | Octadecyl group |
| End-capping: | Methyl group (polymeric end-capping) |
| Target molecular weight: | Below 10kDa |
| pH range: | pH 1.5-9 |
| Maximum pressure: | 250bar / 3,500psi / 25MPa HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa |
| USP Code: | L1 |
Unison UK-C18 is a high-resolution, fast 3μm ODS column that inherits the performance of Cadenza UK-C18. Compared to traditional 5μm particle columns, it offers superior speed and high separation performance.
It demonstrates stable retention characteristics for the separation of highly polar substances using a 100% aqueous mobile phase and also excels in the elution characteristics of low-polarity compounds. Unison UK-C18, which accommodates a wide range of polarities, is the first choice for versatility among IMTAKT ODS columns.
The industry-leading low-pressure 5μm ODS column, Unison US-C18, has the same surface structure as the UK-C18 (3μm) and is a high-performance 5μm column compatible with pharmacopoeia methods.
Low Carryover
Unison UK-C18 demonstrates excellent low carryover performance in LC-MS analysis of pharmaceuticals. This is believed to be due to the development of a different method from conventional packing material synthesis and a novel polymeric end-capping technique.
Reference:
Universal LC-MS Method for Minimized Carryover in a Discovery Bioanalytical Setting
John S. Williams, Stephanie H. Donahue, Hong Gao, Christopher L. Brummel
Bioanalysis (2012) 4(9), 1025-1037
https://www.researchgate.net/publication/225051947_Universal_LC-MS_method_for_minimized_carryover_in_a_discovery_bioanalytical_setting
Technical Information
Differences between Unison UK-C18 and Cadenza CD-C18
- ●Differences in the surface structure of the stationary phase
- ●Differences in basic interactions
- ●Differences in elution characteristics: high polarity region to low polarity region
- ●Differences in elution characteristics: high polarity compounds
- ●Differences in retention behavior due to electrostatic interactions
Column Performance of Unison UK-C18
- ●Column performance with 100% aqueous mobile phase
- ●250x4.6mm (3μm) high-theoretical-plate Unison reversed-phase column
- ●Features of ultra-fast 10mm length columns
- ●Application of ultra-fast 10mm length columns in enzyme reaction analysis
- ●Reproducibility between packing batches
- ●pH durability
- ●Ivermectin and impurities (comparison with ODS column)MS
- ●Ivermectin and impurities (comparison with reversed-phase column)MS
Applications NEW
Latest Applications
- ● [Compounds Related to Sake Brewing] Vinyl Guaiacol MS NEW
- ● [Veterinary Drug] Nitrofuran Antibiotics
- ● [Phenols] Olivetol
- ● beta-Carboline alkaloids
- ● [Redox indicator] Methylene blue
- ● [pH indicator] Congo Red
- ● [Veterinary drugs] Hormones MS
- ● [Cephem antibiotics] Ceftazidime
- ● [Agents for alzheimer-type dementia] Donepezil
- ● [Comparison of Formic Acid and Acetic Acid Mobile Phases] Sesquiterpene Alcohol: Cedrol MS
- ● [Comparison with UK-C8] Sesquiterpene Alcohol: Cedrol MS
- ● [Antiviral] Entecavir
- ● [HCV(hepatitis C virus) protease inhibitor] PSI-6206
- ● [Organic electroluminescent materials] (8-Quinolinolato) lithium
- ● [Sealants / Adhesives] Organic Polysulfide
- ● [Food Additive: Emulsifier] Sucrose fatty acid este
- ● [Organic electroluminescent materials] Organic light-emitting diode green dopant materials
- ● [Organic electroluminescent materials] Organic EL emitting-layer material
- ● [Organic electroluminescent materials] Quinacridone
- ● [Organic electroluminescent materials] Phenanthroline-ruthenium complex
- ● [Organic electroluminescent materials] Diphenylphosphinophenyl ether
- ● [Dye for dye sensitized solar cell] Pyridyl ruthenium complex MS
- ● [Dye for dye sensitized solar cell] Pyridyl ruthenium complex
- ● [HMG-CoA reductase inhibitors] Statins MS
- ● [Potassium-sparing diuretic] Triamterene MS
- ● [Aldosterone antagonist] Canrenoic acid MS
- ● [Hypolipidemic agent] Atorvastatin
- ● [Hypolipidemic agent] Rosuvastatin
- ● [Hypolipidemic agent] Pravastatin
- ● [Antiviral Agents] Idoxuridine
- ● [Antiviral Agents] Penciclovir
- ● [Antiviral Agents] Trifluorothymidine
- ● [Antiviral Agents] Brivudine
- ● [Aldosterone antagonist] Canrenoic acid MS
- ● [Cochineal dye] Carminic acid MS
- ● [Hypolipidemic agent] Gemfibrozil MS
- ● [Antioxidants] Alpha-lipoic acid (Thioctic Acid) MS
- ● [Endocrine Disruptors] Bisphenol A, Diethylstilbestrol MS
- ● [Antiviral Agents] Vidarabine
- ● [Antiviral Agents] Valacyclovir
- ● [Antiviral Agents] Famciclovir
- ● [Antibiotics] Erythromycin analogues MS
- ● [Anti-HIV Agents] Emtricitabine
- ● [Antiviral Drug] Abacavir
- ● [Metronidazolr type antiprotozoal] Metronidazole, tinidazole MS
- ● [Organic Redox-Flow Battery positive electrolyte] TEMPO Free Radical MS
- ● [Monobactam antibiotic] Aztreonam MS
- ● [Dihydrochalcone glycoside] Phlorizin MS
- ● [Pyridine carboxamide pesticides] Flonicamid MS
- ● [5-Nitrofuran antibacterial] Nitrofurantoin MS
- ● [Metronidazole type antiprotozoal] Tinidazole MS
- ● [Metronidazole type antiprotozoal] Metronidazole MS
- ● [Laxative] Sennoside MS
- ● Colistin A B MS
- ● Estrogenic substances MS
- ● Polyether antibiotics MS
- ● Antibiotics - 6 - MS
- ● Antibiotics - 5 - MS
- ● Antibiotics - 4 - MS
- ● Antibiotics - 3 - MS
- ● Antibiotics - 2 - MS
- ● Antibiotics - 1 - MS
- ● [Dyes]Methylene Blue, Leucomalachite Green, Leucogentian Violet MS
- ● [Pesticides] Insecticides - 2 MS
- ● [Pesticides] Insecticides - 1 MS
- ● [Veterinary Drugs] Anticoccidial Agents MS
- ● [Carotenoids] Astaxanthin, Fucoxanthin MS
- ● [Heat-Induced Food Contaminant] Acrylamide MS
- ● [Pyridinecarboxamide Fungicide] Flonicamid Metabolites: TFNA, TFNG MS
- ● [N-Methoxy-pyrazole-carboxamide Fungicide] Pydiflumetofen MS
- ● [Dithiocarbamate Fungicide] Mancozeb MS
- ● [Macrolide Insecticides] Abamectin, Emamectin MS
- ● [Herbicide] 2,4-D (2,4-Dichlorophenoxyacetic Acid) MS
- ● [Hazardous Chemicals] Nitrosamines (N-nitrosamines) MS
- ● [Stevia sweeteners] Stevioside, Rebaudioside MS
- ● [Fungicide] Isothiazolinones MS
- ● [Vitamin D3] Cholecalciferol MS
- ● [Artificial Sweetener] Sucralose MS
- ● [Mycotoxin] Patulin MS
- ● [Preservative] Chlorophenols MS
- ● [Coffee compounds] Chlorogenic acid MS
- ● [Antiglaucoma drug] Pilocarpine
- ● [Alcoholic beta-glucoside] Salicin
- ● [Coenzyme form of Vitamin B12] Methylcobalamin
- ● [Antifungal agent] Griseofulvin
- ● [Cyanogenic glycoside] Amygdalin
- ● [Alkaloid] Camptothecin
- ● [Isocoumarin derivatives] Bergenin
- ● [Coumarin anticoagulant] Dicoumarol
- ● [Furanochromone, Vasodilator] Khellin
- ● [Phytoestrogens] Isoflavones
- ● [Indole alkaloid] Gramine
- ● [Histology Stains] Hematoxylin
- ● [beta-Lactam antibiotic] Oxacillin
- ● [Lignan derivatives] Nordihydroguaiaretic Acid
- ● [Near infrared fluorescent dye] Puerarin Indocyanine Green
- ● [Isoflavone glycoside] Puerarin
- ● [Resveratrol Glycoside] Piceid
- ● Daidzein (Soybean isoflavone)
- ● [Synthetic Stimulant] Adrafinil MS
- ● [Tyrosinase inhibitor] Rhododendrol
- ● [Sesame lignan] Sesamol
- ● Musty odor compounds in drinking water MS
- ● Amino Naphthol Sulfonic Acid Isomers MS
- ● [Pulmonary Hypertension drug] Bosentan, Macitentan MS
- ● [Pulmonary Hypertension drug] Bosentan, Macitentan
- ● Cephalosporin Antibiotics MS
- ● Red Yeast Extract (Lovastatin, Monacolin K)
- ● Melatonin-Related Compounds MS
- ● Melatonin-Related Compounds
- ● [Antirheumatic] Sulfasalazine
- ● [Flavonoid Glycoside] Rutin
- ● 3,3''-Diaminobenzidine
- ● [Isomers] Quinoline, Isoquinoline
- ● [Heme Metabolite] Bilirubin
- ● [Glutarimide Antibiotic] Cycloheximide MS
- ● [Glutarimide Antibiotic] Cycloheximide
- ● [Fatty Acid Isomers] Isostearic Acid, Stearic Acid
- ● [Pyrine Antipyretic Analgesic] Antipyrine (Neutral Mobile Phase)
- ● [Pyrine Antipyretic Analgesic] Antipyrine (Acidic Mobile Phase)
- ● [Rubber Antioxidants - Amine Antioxidants] PPDs
- ● [Peripheral Vasodilator] Ifenprodil
- ● [Vascular Protective Drug] Troxerutin
- ● [Hemostatic Agent] Carbazochrome Sulfonate
- ● [Hyperlipidemia Treatment] Pemafibrate
- ● [Antihypertensive] Olmesartan Medoxomil
- ● [Vasodilator] Trimetazidine MS
- ● [Pesticide] Organophosphorus Insecticides 3 MS
- ● [Pesticide] Organophosphorus Insecticides 2 MS
- ● [Pesticide] Organophosphorus Insecticides 1 MS
- ● [Anticoagulant] Warfarin
- ● [Pesticide] Neonicotinoid Insecticides MS
- ● [Antineoplastic Agent] Paclitaxel MS
- ● [Anti-ulcer Drug] Rebamipide
- ● [Antithrombotic Drug] Edoxaban
- ● [Antiplatelet Drug] Clopidogrel
- ● [Pesticide] Acephate MS
- ● Unapproved food colorings (14 types)
- ● Food additives (12 food colorings)
- ● [Stimulant Laxative] Picosulfate
- ● [Quinolone Antibiotic] Oxolinic Acid
- ● [Anti-inflammatory Drug] Glycyrrhizic Acid
- ● [Osteoporosis Treatment] Raloxifene
- ● [Diabetes Treatment] Pioglitazone
- ● [Antimalarial Drug, Herbal Medicine] Artemisinin and Its Derivatives MS
- ● [Analgesic] Zaltoprofen
- ● [Anxiolytic, GABA-A Receptor Agonist] Clotiazepam
- ● [Asthma Treatment, Leukotriene Receptor Antagonist] Pranlukast
- ● [Antiviral Drug] Remdesivir - Provided by Professor Tsuji, Nihon University
- ● [ED Treatment] Comparative Dissolution of Tadalafil (PDE5 Inhibitor)
- ● [Cough Suppressant and Expectorant] Tipepidine Hibenzate
- ● [Smoking Cessation Aid] Varenicline
- ● Catechins in Tea Beverages
- ● Oligonucleotide Analysis Without Ion-Pair Reagents
- ● Comparative Dissolution of Omega-3 Fatty Acids (DHA, EPA, AA)
- ● Comparative Dissolution of Omega-3 Fatty Acid Esters (Ethyl-DHA, Ethyl-EPA)
User Reports
-
●USDA (United States Department of Agriculture)
LC-MS/MS Analysis of Water-Soluble Vitamins
- ●Perrigo Company of South Carolina Inc., USA
- ●Massachusetts State Crime Lab, USA
-
●Agilent Technologies Inc.
LC-MS/MS Analysis of Haloacetic Acids According to Official Methods
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●Kyoto Prefectural Police Department Forensic Science Laboratory
LC-MS/MS Analysis of Three Active Ingredients in ED Drugs
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●Public Health Institute of Kobe City
LC-MS/MS Analysis of Three Active Ingredients in ED Drugs
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●Yamaguchi Prefectural Environmental Health Center
LC-MS/MS Analysis of α-Solanine and α-Chaconine
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●Tokushima Prefectural Industrial Technology Center
Rapid Analysis of Plant Pigments in Salted Mizuna
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●JEOL Ltd.
High-Sensitivity Analysis by LC-TOFMS (AccuTOF) with Excellent Quantification
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●Takara Bio Inc.
Overwhelming Difference in Column Injection Volume
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●Kyushu Industrial Health Association
Analysis of Methamidophos and Dichlorvos