PRODUCTS Unison Columns Unison UK-C18
UPDATE: New information has been added to the Applications tab
IMTAKT HPLC COLUMN UNISON UK-C18

From aqueous to non-aqueous phases, high-resolution ODS column

IMTAKT HPLC COLUMN UNISON UK-C18

FEATURES

World's highest theoretical plate number of 50,000 plates column (250x4.6mm)

Wide selectivity range from 100% aqueous to non-aqueous mobile phases

Unique end-capping: basic, acid-resistant, alkali-resistant

Wide range of column sizes: inner diameter 0.075 - 28mm / length 10-500mm

High-pressure specifications options
(HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa)

High-pressure metal-free column (MF: 50MPa)

MAIN SPECIFICATIONS

Particle size: 3μm (Unison UK-C18)
5μm (Unison US-C18)
Base material: Fully porous high-purity silica gel
Pore size: 13nm
Ligand: Octadecyl group
End-capping: Methyl group (polymeric end-capping)
Target molecular weight: Below 10kDa
pH range: pH 1.5-9
Maximum pressure: 250bar / 3,500psi / 25MPa
HP: 500bar / 7,500psi / 50MPa,
UP: 1,000bar / 15,000psi / 100MPa
USP Code: L1

Unison UK-C18 is a high-resolution, fast 3μm ODS column that inherits the performance of Cadenza UK-C18. Compared to traditional 5μm particle columns, it offers superior speed and high separation performance.

It demonstrates stable retention characteristics for the separation of highly polar substances using a 100% aqueous mobile phase and also excels in the elution characteristics of low-polarity compounds. Unison UK-C18, which accommodates a wide range of polarities, is the first choice for versatility among IMTAKT ODS columns.

The industry-leading low-pressure 5μm ODS column, Unison US-C18, has the same surface structure as the UK-C18 (3μm) and is a high-performance 5μm column compatible with pharmacopoeia methods.

Low Carryover

Unison UK-C18 demonstrates excellent low carryover performance in LC-MS analysis of pharmaceuticals. This is believed to be due to the development of a different method from conventional packing material synthesis and a novel polymeric end-capping technique.

Reference:
Universal LC-MS Method for Minimized Carryover in a Discovery Bioanalytical Setting
John S. Williams, Stephanie H. Donahue, Hong Gao, Christopher L. Brummel
Bioanalysis (2012) 4(9), 1025-1037
https://www.researchgate.net/publication/225051947_Universal_LC-MS_method_for_minimized_carryover_in_a_discovery_bioanalytical_setting

[Differences between Unison UK-C18 and Cadenza CD-C18]

[Column Performance of Unison UK-C18]

Related Info