Aqueous and Non-Aqueous, High-Performance Silica Column
FEATURES
Normal-phase column usable for both aqueous and non-aqueous systems
High-resolution 3μm silica
Wide range of column sizes: inner diameter 0.075 - 28mm / length 10-500mm
High-pressure resistance options
(HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa)
MAIN SPECIFICATIONS
| Particle size: | 3μm (Unison UK-Silica) 5μm (Unison US-Silica) |
| Base material: | Fully porous high-purity silica gel |
| Pore size: | 13nm |
| Ligand: | Silica |
| Target molecular weight: | Below 10kDa |
| pH range: | pH 1-7 |
| Maximum pressure: | 250bar / 3,500psi / 25MPa HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa |
| USP Code: | L3 |
Unison UK series with 3μm particles, which offers higher resolution compared to conventional 5μm columns, is also highly effective in normal-phase separations with a silica stationary phase. Unison UK-Silica was developed to achieve higher separation and faster analyses than traditional 5μm, 250mm silica columns. Unison UK-Silica can be used for both non-aqueous and aqueous normal-phase separations.
Unison UK-Silica columns, when used with acidic additives such as acetic acid or formic acid, are fundamental normal-phase columns that are expected to yield significant results in various fields such as natural product chemistry and organic synthesis chemistry.
CAUTION
UK-Silica is a pure silica column. While silica is extremely robust under acidic conditions, it is prone to dissolution in neutral pH environments such as ammonium acetate, which raises concerns about column bleeding and durability in LC-MS applications.
When using silica columns with aqueous mobile phases containing neutral salts like ammonium acetate or ammonium formate, it is recommended to keep the mixture ratio below 20% (preferably below 10%) to avoid silica hydrolysis.
Technical Information
Applications NEW
- ● Trifluorophenylboronic acid(Condensation organocatalysts) MS NEW
- ● Guanidine hydrochloride(protein denaturant) MS NEW
- ● Guanidine hydrochloride(protein denaturant)
- ● Xylenol isomers MS
- ● Urea, allantoin MS
- ● Ethylene glycols MS
- ● Valganciclovir (Antiviral)
- ● 5-Nitrofurfural (Aromatic aldehyde)
- ● Lithium Ion Battery Electrolytes and Additives MS
- ● Sphingoglycolipids (Galactocerebrosides)
- ● Biguanide Oral Antidiabetic Agents (Metformin)
- ● Normal-phase and reversed-phase separation of pantothenic acid and ascorbic acid
- ● Normal-phase and reversed-phase separation of antioxidants (Irgafos, Irganox)
- ● Normal-phase and reversed-phase separation of influenza drug (zanamivir)
- ● Separation of Eye Drops Components (Allantoin, Chlorpheniramine) - acidic pH
- ● Separation of Preservatives (Dehydroacetic Acid, Sorbic Acid, Benzoic Acid) (Normal-phase Comparison)
- ● Separation of Tocopherols and Tocotrienols (Normal-phase Comparison)
- ● Lipid A (Salmonella minnesota Re595)